中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (24): 2182-2188.doi: 10.4103/1673-5374.147951

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

高压氧治疗脊髓损伤的作用在于改善了损伤局部的微环境

  

  • 收稿日期:2014-11-20 出版日期:2014-12-25 发布日期:2014-12-25

Hyperbaric oxygen therapy improves local microenvironment after spinal cord injury

Yang Wang 1, Shuquan Zhang 2, Min Luo 1, Yajun Li 3   

  1. 1 Department of Orthopedics, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
    2 Department of Orthopedics, Nankai Hospital, Tianjin, China
    3 School of Mathematics, Jilin University, Changchun, Jilin Province, China
  • Received:2014-11-20 Online:2014-12-25 Published:2014-12-25
  • Contact: Yajun Li, School of Mathematics, Jilin University, Changchun 130028, Jilin Province, China, yanyao523@163.com.
  • Supported by:

    This study was financially supported by grants from the Science and Technology
    Development Project of Jilin Province in China, No. 20110492.

摘要:

临床研究证实高压氧治疗能有效改善脊髓损伤患者的运动能力。为了进一步研究高压氧干预对脊髓损伤后神经功能恢复的作用机制,实验按照改良的自由落体重物打击法建立大鼠急性脊髓损伤模型,4h后以0.2 MPa的高压氧进行治疗,4次/d,连续3d。结果显示,经高压氧治疗的急性脊髓损伤大鼠脊髓组织中凋亡细胞的数量明显减少,水通道蛋白4/9 mRNA和蛋白表达降低,NF-200阳性神经纤维数量增加,脊髓空间减小,后肢运动功能明显增强,体感诱发电位和运动诱发电位电生理功能恢复明显。表明高压氧治疗通过减少大鼠脊髓损伤区细胞凋亡和降低水通道蛋白4/9基因和蛋白的表达,从而有效改善脊髓损伤局部再生的微环境,对损伤脊髓起到了保护和修复的作用。

关键词: 神经再生, 脊髓损伤, 高压氧, 神经再生, 运动功能, 大鼠, 微环境, 水通道蛋白4, 水通道蛋白9

Abstract:

Clinical studies have shown that hyperbaric oxygen therapy improves motor function in patients with spinal cord injury. In the present study, we explored the mechanisms associated with the recovery of neurological function after hyperbaric oxygen therapy in a rat model of spinal cord injury. We established an acute spinal cord injury model using a modification of the free-falling object method, and treated the animals with oxygen at 0.2 MPa for 45 minutes, 4 hours after injury. The treatment was administered four times per day, for 3 days. Compared with model rats that did not receive the treatment, rats exposed to hyperbaric oxygen had fewer apoptotic cells in spinal cord tissue, lower expression levels of aquaporin 4/9 mRNA and protein, and more NF-200 positive nerve fibers. Furthermore, they had smaller spinal cord cavities, rapid recovery of somatosensory and motor evoked potentials, and notably better recovery of hindlimb motor function than model rats. Our findings indicate that hyperbaric oxygen therapy reduces apoptosis, downregulates aquaporin 4/9 mRNA and protein expression in injured spinal cord tissue, improves the local microenvironment for nerve regeneration, and protects and repairs the spinal cord after injury.

Key words: nerve regeneration, spinal cord injury, hyperbaric oxygen, motor function, rats, microenvironment, aquaporin 4, aquaporin 9, neural regeneration