中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (3): 236-242.doi: 10.4103/1673-5374.128214

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

660nm红光增强骨髓间充质干细胞治疗缺氧缺血性脑损伤的效果

  

  • 出版日期:2014-02-15 发布日期:2014-02-15
  • 基金资助:

    国家自然科学基金项目(30970758,31271060),国家科技支撑计划课题(2011BAI14B04,2012BAI16B02),重庆市自然科学基金项目(No. cstc2012jjA10103)

660 nm red light-enhanced bone marrow mesenchymal stem cell transplantation for hypoxic-ischemic brain damage treatment

Xianchao Li1, Wensheng Hou1, 2, Xiaoying Wu1, 2, Wei Jiang3, Haiyan Chen2, Nong Xiao3, Ping Zhou2   

  1. 1 Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing, China
    2 Chongqing Engineering Research Center for Medical Electronics Technology, Chongqing, China
    3 Rehabilitation Center, Children’s Hospital of Chongqing Medical University, Chongqing, China
  • Online:2014-02-15 Published:2014-02-15
  • Contact: Xiaoying Wu, M.D., Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China; Chongqing Engineering Research Center for Medical Electronics Technology, Chongqing 400044, China, xiaoyingwu69@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 30970758, 31271060; the National Science and Technology Support Program of China, No. 2011BAI14B04, 2012BAI16B02; the Natural Science Foundation of Chongqing in China, No. cstc2012jjA10103.

摘要:

骨髓间充质干细胞移植治疗新生儿缺氧缺血性脑损伤的价值已得到肯定,但存在体内移植效率和体内存活定植分化率低的问题。而波长在600-1000nm范围的红光或近红外光有促进细胞迁移和抗细胞凋亡的作用,将其与骨髓间充质干细胞联用治疗缺氧缺血性脑损伤是否更好呢?实验运用Transwell法检测上层培养的骨髓间充质干细胞定向向下层的OGD损伤的原代神经元迁移定植情况,发现绿色荧光标记的骨髓间充质干细胞经波长660nm、光功率密度为60mW/cm2发光二极管红光连续照射40h,骨髓间充质干细胞向缺氧缺血性损伤的原代神经元迁移数量逐步增加。同时,实验给予缺氧缺血性脑损伤模型新生7d乳鼠,腹腔注射106个骨髓间充质干细胞,并以660nm、60mW/cm2发光二极管红光连续头部照射7d。穿梭箱测试结果显示红光联合骨髓间充质干细胞治疗的缺氧缺血损伤大鼠的主动逃避率显著升高,且高于单纯骨髓间充质干细胞。提示660nm发光二极管红光照射通过增强骨髓间充质干细胞迁移能力,进而提高其移植治疗缺氧缺血脑损伤的效果。

关键词: 神经再生, 干细胞, Transwell试验, 红光治疗, 缺氧缺血性脑损伤, 骨髓间充质干细胞, 定向移植, 细胞迁移, 学习能力, 基金资助文章

Abstract:

Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hypoxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and differentiation efficiencies are relatively low. Red or near-infrared light from 600–1,000 nm promotes cellular migration and prevents apoptosis. Thus, we hypothesized that the combination of red light with bone marrow mesenchymal stem cell transplantation would be effective for the treatment of hypoxic-ischemic brain damage. In this study, the migration and colonization of cultured bone marrow mesenchymal stem cells on primary neurons after oxygen-glucose deprivation were detected using Transwell assay. The results showed that, after a 40-hour irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2, an increasing number of green fluorescence-labeled bone marrow mesenchymal stem cells migrated towards hypoxic-ischemic damaged primary neurons. Meanwhile, neonatal rats with hypoxic-ischemic brain damage were given an intraperitoneal injection of 1 × 106 bone marrow mesenchymal stem cells, followed by irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2 for 7 successive days. Shuttle box test results showed that, after phototherapy and bone marrow mesenchymal stem cell transplantation, the active avoidance response rate of hypoxic-ischemic brain damage rats was significantly increased, which was higher than that after bone marrow mesenchymal stem cell transplantation alone. Experimental findings indicate that 660 nm red light emitting diode irradiation promotes the migration of bone marrow mesenchymal stem cells, thereby enhancing the contribution of cell transplantation in the treatment of hypoxic-ischemic brain damage.

Key words: nerve regeneration, stem cells, Transwell assay, red light, hypoxic-ischemic brain damage, bone marrow mesenchymal stem cells, transplantation, migration, learning ability, NSFC grant, neural regeneration