中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (3): 318-328.doi: 10.4103/1673-5374.128231

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

短期丰富环境训练诱导前额叶皮质DCX阳性细胞的增殖和成熟

  

  • 收稿日期:2013-11-02 出版日期:2014-02-15 发布日期:2014-02-15
  • 基金资助:

    国家自然科学基金项目(30900773),湖南省自然科学基金(11JJ2020)

Short-term environmental enrichment exposure induces proliferation and maturation of  doublecortin-positive cells in the prefrontal cortex

Chunling Fan1, Mengqi Zhang2, Lei Shang1, Ngobe Akume Cynthia3, Zhi Li4, Zhenyu Yang4, Dan Chen1, Jufang Huang1, Kun Xiong1   

  1. 1 Department of Anatomy and Neurobiology, Central South University School of Basic Medical Sciences, Changsha, Hunan Province, China
    2 Grade 2006, Eight-year Medicine Doctor Program, Central South University Xiangya School of Medicine, Changsha, Hunan Province, China
    3 Grade 2011, Six-year Medicine Program of International Student, Central South University Xiangya School of Medicine, Changsha, Hunan
    Province, China
    4 Grade 2008, Eight-year Medicine Doctor Program, Central South University Xiangya School of Medicine, Changsha, Hunan Province, China
  • Received:2013-11-02 Online:2014-02-15 Published:2014-02-15
  • Contact: Kun Xiong, M.D., Department of Anatomy and Neurobiology, Central South University School of Basic Medical Sciences, Changsha 410013, Hunan Province, China, xiongkun2001@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 30900773; the Natural Science Foundation of Hunan Province in China, No. 11JJ2020; and Young Teachers Training Program of University of Hunan Province.

摘要:

本课题组前期研究已发现,在一些成年哺乳动物如豚鼠的前额叶皮质浅层持续有大量的不成熟神经元标记物DCX阳性细胞存在,它们在正常生理条件下有微弱的自我更新能力。丰富环境训练是否对其前额叶新皮质存在的不成熟神经元的增殖及成熟有影响呢?实验用短期丰富环境训练健康成年豚鼠,即动物每日在配有各种训练玩具的空间内活动2次,60 min/次,持续2周。免疫荧光染色结果显示,经过短期丰富环境训练后的豚鼠前额叶新皮质第Ⅱ层不成熟神经元标记物DCX阳性表达细胞数量显著增加;同时,这些DCX阳性细胞同时共表达细胞增殖标记物5-溴-2-脱氧脲苷和细胞活性标记物的c-Fos蛋白,及成熟神经元标记物NeuN。结果说明,短期丰富环境刺激可以诱导成年豚鼠前额叶新皮质第Ⅱ层新生细胞的增殖、活化和成熟。

关键词: 神经再生, 神经发生, 额叶前皮质, 新皮质, 豚鼠, 双皮质蛋白, 细胞增殖, 神经元, 溴脱氧尿苷, 国家自然科学基金

Abstract:

Previous studies have demonstrated that doublecortin-positive immature neurons exist predominantly in the superficial layer of the cerebral cortex of adult mammals such as guinea pigs, and these neurons exhibit very weak properties of self-proliferation during adulthood under physiological conditions. To verify whether environmental enrichment has an impact on the proliferation and maturation of these immature neurons in the prefrontal cortex of adult guinea pigs, healthy adult guinea pigs were subjected to short-term environmental enrichment. Animals were allowed to play with various cognitive and physical stimulating objects over a period of 2 weeks, twice per day, for 60 minutes each. Immunofluorescence staining results indicated that the number of doublecortin-positive cells in layer II of the prefrontal cortex was significantly increased after short-term environmental enrichment exposure. In addition, these doublecortin-positive cells co-expressed 5-bromo-2-deoxyuridine (a marker of cell proliferation), c-Fos (a marker of cell viability) and NeuN (a marker of mature neurons). Experimental findings showed that short-term environmental enrichment can induce proliferation, activation and maturation of doublecortin-positive cells in layer II of the prefrontal cortex of adult guinea pigs.

Key words: nerve regeneration, neurogenesis, prefrontal cortex, neocortex, guinea pig, doublecortin protein, cell proliferation, neurons, 5-bromodeoxyuridine, NSFC grant, neural regeneration