中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (3): 541-548.doi: 10.4103/1673-5374.228760

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

三焦针法与黄地散提升神经干细胞移植后SAMP8小鼠行为能力和海马突触素的表达

  

  • 收稿日期:2017-10-18 出版日期:2018-03-15 发布日期:2018-03-15
  • 基金资助:

    中国国家自然科学基金项目（17JCYBJC26200，12JCQNJC07400），天津市公共卫生科基金项目（2014KY15），高等教育博士专项基金（20121210120002）

Combined acupuncture and HuangDiSan treatment affects behavior and synaptophysin levels in the hippocampus of senescence-accelerated mouse prone 8 after neural stem cell transplantation

Chun-lei Zhou1, Lan Zhao2, 3 , Hui-yan Shi2, 3, Jian-wei Liu4, Jiang-wei Shi2, Bo-hong Kan2, 3, Zhen Li2, 3, Jian-chun Yu2, Jing-xian Han2   

  1. 1 Tianjin First Center Hospital, Tianjin, China
    2 First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China
    3 Tianjin Key Laboratory of Acupuncture and Moxibustion, Tianjin, China
    4 Tianjin University of Traditional Chinese Medicine, Tianjin, China
  • Received:2017-10-18 Online:2018-03-15 Published:2018-03-15
  • Contact: Lan Zhao, Ph.D.,lanzhao69@163.com.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81202740 and 81603686; the Natural Science Foundation of Tianjin of China, No. 17JCYBJC26200 and 12JCQNJC07400; the Public Health Bureau Science and Technology Foundation of Tianjin of China, No. 2014KY15; and the Specialized Research Foundation for the Doctoral Program of Higher Education, No. 20121210120002

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摘要：

研究显示用三焦针法及黄地散治疗可促进SAMP8小鼠外源性神经干细胞的增殖、迁移和分化，改善痴呆小鼠学习记忆障碍及行为学功能。因此作者拟观察三焦针法与黄地散是否能通过提高神经干细胞移植治疗阿尔茨海默病模型小鼠的效果。实验在SAMP8小鼠海马齿状回移植5×105神经干细胞前后的各15d内，采用三焦针法刺激膻中、中脘、气海以及双侧血海和双侧足三里，同时每天灌胃0.2mL以地黄、当归等制成的黄地散。结果显示，与单纯进行神经干细胞移植的小鼠相比，同时进行三焦针法与黄地散灌胃的小鼠学习记忆能力改善更为明显，海马突触素蛋白及基因表达增加幅度更大，故可说明三焦针法联合黄地散灌胃具有改善小鼠的痴呆症状，作用途径与上调突触素表达有关。

orcid:0000-0002-7449-2947(Lan Zhao)

 

关键词: 神经再生, 神经元, 神经退行性疾病, 老年痴呆, 微环境, 中医药, 行为, 神经干细胞移植, 突触素

Abstract:

Sanjiao acupuncture and HuangDiSan can promote the proliferation, migration and differentiation of exogenous neural stem cells in senescence-accelerated mouse prone 8 (SAMP8) mice and can improve learning and memory impairment and behavioral function in dementia-model mice. Thus, we sought to determine whether Sanjiao acupuncture and HuangDiSan can elevate the effect of neural stem cell transplantation in Alzheimer’s disease model mice. Sanjiao acupuncture was used to stimulate Danzhong (CV17), Zhongwan (CV12),Qihai (CV6), bilateral Xuehai (SP10) and bilateral Zusanli (ST36) 15 days before and after implantation of neural stem cells (5 × 105) into the hippocampal dentate gyrus of SAMP8 mice. Simultaneously, 0.2 mL HuangDiSan, containing Rehmannia Root and Chinese Angelica,was intragastrically administered. Our results demonstrated that compared with mice undergoing neural stem cell transplantation alone,learning ability was significantly improved and synaptophysin mRNA and protein levels were greatly increased in the hippocampus of mice undergoing both Sanjiao acupuncture and intragastric administration of HuangDiSan. We conclude that the combination of Sanjiao acupuncture and HuangDiSan can effectively improve dementia symptoms in mice, and the mechanism of this action might be related to the regulation of synaptophysin expression.

Key words: nerve regeneration, neurons, neurodegeneration, Alzheimer’s disease, microenvironment, Chinese medicine, behavior, neural stem cell transplantation, synaptophysin, neural regeneration