中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (5): 792-796.doi: 10.4103/1673-5374.156984

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

Ras/Raf/ERK1/2信号通路表达上调:参与损伤脊髓修复的新希望?

  

  • 收稿日期:2015-04-16 出版日期:2015-05-15 发布日期:2015-05-15

Upregulated Ras/Raf/ERK1/2 signaling pathway: a new hope in the repair of spinal cord injury

Tao Liu, Fu-jiang Cao, Dong-dong Xu, Yun-qiang Xu, Shi-qing Feng   

  1. Department of Orthopedics, General Hospital of Tianjin Medical University, Tianjin, China
  • Received:2015-04-16 Online:2015-05-15 Published:2015-05-15
  • Contact: Shi-qing Feng, M.D. Ph.D., caofujiang@126.com.
  • Supported by:

    This work was funded by the National Natural Science Foundation for Young Scholars of China, No. 81101362 and 81401784; and the Key Project of National Natural Science Foundation of China, No. 81330042. 

摘要:

研究显示Ras/Raf/ERK1/2信号通路具有促进神经细胞凋亡的功能。因此我们提出Ras/Raf/ERK1/2信号通路可能在大鼠损伤脊髓中被异常调节的假设。实验以打击法建立T9脊髓损伤大鼠模型,Western blot和免疫组化染色结果显示,损伤脊髓中Ras蛋白表达增加,Raf激酶A-Raf,B-Raf和C-Raf磷酸化水平明显增加;而且Ras/Raf信号激酶MEK1/2和ERK1/2磷酸化水平增加。表明大鼠损伤脊髓中Ras/Raf/ERK1/2信号通路活性上调,其可能参与损伤脊髓的修复。

关键词: 神经再生, Ras/Raf/Erk1/2信号通路, 脊髓损伤, 细胞凋亡, 修复, 调节, 抑制

Abstract:

An increasing number of studies report that the Ras/Raf/extracellular signal-regulated kinase 1/2 (ERK1/2) signaling pathway has a death-promoting apoptotic function in neural cells. We hypothesized that the Ras/Raf/ERK1/2 signaling pathway may be abnormally regulated in rat injured spinal cord models. The weight drop method was used to establish rat spinal cord injury at T9. Western blot analysis and immunohistochemical staining revealed Ras expression was dramatically elevated, and the phosphorylations of A-Raf, B-Raf and C-Raf were all upregulated in the injured spinal cord. Both mitogen-activated protein kinase kinase 1/2 and ERK1/2, which belong to the Ras/Raf signaling kinases, were upregulated. These results indicate that Ras/Raf/ERK1/2 signaling may be upregulated in injured spinal cord and are involved in recovery after spinal cord injury.

Key words: nerve regeneration, Ras/Raf/Erk1/2 signaling pathway, spinal cord injury, apoptosis, repair, regulation, inhibition, neural regeneration