中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (7): 1147-1152.doi: 10.4103/1673-5374.156983

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

小鼠脊髓损伤后神经元内microRNA-124表达的缺失可反映脊髓损伤状态

  

  • 收稿日期:2015-06-02 出版日期:2015-07-24 发布日期:2015-07-24
  • 基金资助:

    国家自然科学基金(81371364)

Loss of microRNA-124 expression in neurons in the peri-lesion area in mice with spinal cord injury

Yu Zhao 1, Hui Zhang 1, Dan Zhang 2, Cai-yong Yu 3, Xiang-hui Zhao 3, Fang-fang Liu 3, Gan-lan Bian 3, Gong Ju 3, Jian Wang 3   

  1. 1 Department of Anatomy, Hebei North University, Zhangjiakou, Hebei Province, China
    2 Department of Stomatology, the First Hospital of Zhangjiakou, Zhangjiakou, Hebei Province, China
    3 Institute of Neurosciences, the Fourth Military Medical University, Xi’an, Shaanxi Province, China
  • Received:2015-06-02 Online:2015-07-24 Published:2015-07-24
  • Contact: Gong Ju or Jian Wang, jugong@fmmu.edu.cn or jwangfm@163.com.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81371364.

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摘要：

microRNA-124广泛表达于哺乳动物中枢神经系统的神经元内，在胚胎期神经发育以及发育后期神经分化的基因表达调控过程中起着重要作用，但在脊髓损伤后的表达谱及其作用机制目前尚未阐明。我们通过原位杂交实验检测了microRNA-124在小鼠脑和脊髓的表达分布。通过实时定量PCR实验检测了microRNA-124在小鼠脊髓损伤后1，3，7 d的表达变化，并运用microRNA-124原位杂交与NeuN免疫组织化学双重染色方法观察了脊髓损伤区神经元microRNA-124的分布。结果显示microRNA-124广泛分布于脑和脊髓的神经元内，脊髓损伤后7 d内神经元内microRNA-124的表达显著下降，脊髓损伤区远侧区域神经元可见NeuN与microRNA-124共表达，而在损伤区周围NeuN表达阳性的神经元内microRNA-124却并不表达。根据这些，作者认为神经元中microRNA-124的表达缺失与脊髓损伤进程密切相关。

关键词: 神经再生, 脊髓损伤, 微小RNA, 脊髓, 原位杂交, 免疫组织化学, 地高辛, NeuN蛋白, 脑, 神经可塑性, 修复, 凋亡, 国家自然科学基金

Abstract:

MicroRNA-124 (miR-124) is abundantly expressed in neurons in the mammalian central nervous system, and plays critical roles in the regulation of gene expression during embryonic neurogenesis and postnatal neural differentiation. However, the expression profile of miR-124 after spinal cord injury and the underlying regulatory mechanisms are not well understood. In the present study, we examined the expression of miR-124 in mouse brain and spinal cord after spinal cord injury using in situ hybridization. Furthermore, the expression of miR-124 was examined with quantitative RT-PCR at 1, 3 and 7 days after spinal cord injury. The miR-124 expression in neurons at the site of injury was evaluated by in situ hybridization combined with NeuN immunohistochemical staining. The miR-124 was mainly expressed in neurons throughout the brain and spinal cord. The expression of miR-124 in neurons significantly decreased within 7 days after spinal cord injury. Some of the neurons in the peri-lesion area were NeuN+/miR-124−. Moreover, the neurons distal to the peri-lesion site were NeuN+/miR-124+. These findings indicate that miR-124 expression in neurons is reduced after spinal cord injury, and may reflect the severity of spinal cord injury.

Key words: nerve regeneration, spinal cord injury, microRNA, spinal cord, in situ hybridization, immunohistochemistry, digoxin, NeuN protein, brain, neural plasticity, repair, apoptosis, NSFC grants, neural regeneration