中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (4): 744-749.doi: 10.4103/1673-5374.295346

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

脊髓损伤小鼠大脑运动皮质突触的重塑

  

  • 出版日期:2021-04-15 发布日期:2020-12-21
  • 基金资助:

    中国技术强基计划项目(2019-JCJQ-JJ-149);解放军总医院军事医学青年项目(QNF19056

Synaptic remodeling in mouse motor cortex after spinal cord injury

Ke-Xue Zhang1, #, Jia-Jia Zhao2, #, Wei Chai3, *, Ji-Ying Chen3, *   

  1. 1 Department of Pediatric Surgery, Chinese PLA General Hospital, Beijing, China;  2 Department of Anesthesiology, Shunyi District Hospital, Beijing, China; 3Department of Orthopedics, Chinese PLA General Hospital, Beijing, China
  • Online:2021-04-15 Published:2020-12-21
  • Contact: Ji-Ying Chen, MD, 570457036@qq.com; Wei Chai, MD, chaiwei301@163.com.
  • Supported by:
    This work was supported by grants of Foundation and Strengthening Program in Technical Area of China, No. 2019-JCJQ-JJ-149 (to KXZ); and Military Medical Youth Program of Chinese PLA General Hospital, No. QNF19056 (to KXZ).

摘要:

脊髓损伤可严重阻碍中枢神经系统和周围神经系统间的信息交换,且脊髓损伤后运动皮质中突触的命运也未少有研究。为探索脊髓损伤后大脑运动皮质中突触重组的情况,实验建立了T12脊髓半切小鼠模型,通过磨薄颅骨结合双光子显微成像技术研究观察活体小鼠大脑运动皮质锥体神经元中突触后树突棘和突触前轴突。结果发现,脊髓半切可导致小鼠大脑双侧运动皮质中树突棘的重塑,且随时间的延长,重塑区域会发生变化。脊髓损伤会使大脑双侧皮质中稳定树突棘变得不稳定,且退化的树突棘更加难以重生,对侧运动皮质中新生树突棘则明显增加,但其存活率很低,即新生树突棘很脆弱;但突触前轴突没有明显的变化。结果说明髓损伤小鼠的上游神经中枢大脑运动皮质可在突触水平发生重塑,且重塑主要发生在突触的后组成部分而非前组成部分。实验于2015年4月16日经解放军总医院伦理委员会批准,批准号201504168S。

https://orcid.org/0000-0001-8166-4658 (Ji-Ying Chen); 

https://orcid.org/0000-0001-8781-9745 (Wei Chai); 

https://orcid.org/0000-0002-6419-2553 (Ke-Xue Zhang)

关键词: 中枢神经系统, 脊髓, 损伤, 修复, 保护, 再生, 可塑性, 突触

Abstract: Spinal cord injury dramatically blocks information exchange between the central nervous system and the peripheral nervous system. The resulting fate of synapses in the motor cortex has not been well studied. To explore synaptic reorganization in the motor cortex after spinal cord injury, we established mouse models of T12 spinal cord hemi-section and then monitored the postsynaptic dendritic spines and presynaptic axonal boutons of pyramidal neurons in the hindlimb area of the motor cortex in vivo. Our results showed that spinal cord hemi-section led to the remodeling of dendritic spines bilaterally in the motor cortex and the main remodeling regions changed over time. It made previously stable spines unstable and eliminated spines more unlikely to be re-emerged. There was a significant increase in new spines in the contralateral motor cortex. However, the low survival rate of the new spines demonstrated that new spines were still fragile. Observation of presynaptic axonal boutons found no significant change. These results suggest the existence of synapse remodeling in motor cortex after spinal cord hemi-section and that spinal cord hemi-section affected postsynaptic dendritic spines rather than presynaptic axonal boutons. This study was approved by the Ethics Committee of Chinese PLA General Hospital, China (approval No. 201504168S) on April 16, 2015.

Key words: central nervous system, plasticity, recovery, regeneration, repair, spinal cord injury, synapses