中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (16): 1445-1454.doi: 10.3969/j.issn.1673-5374.2013.16.001

• 原著:脑损伤修复保护与再生 •    下一篇

运动再学习疗法为何可促进恒河猴脑缺血后神经功能恢复？

  

  • 收稿日期:2012-12-30 修回日期:2013-04-07 出版日期:2013-06-05 发布日期:2013-06-05

How does the motor relearning program improve neurological function of brain ischemia monkeys?

Yong Yin1, Zhen Gu2, Lei Pan1, Lu Gan1, Dongdong Qin3, Bo Yang4, Jin Guo5, Xintian Hu3, Tinghua Wang6, Zhongtang Feng6   

  1. 1 Department of Rehabilitation Medicine, Fourth Hospital of Kunming Medical University, Kunming 650021, Yunnan Province, China
    2 Department of Neurosurgery, Fourth Hospital of Kunming Medical University, Kunming 650021, Yunnan Province, China
    3 Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan Province, China
    4 Department of Nuclear Medicine, Fourth Hospital of Kunming Medical University, Kunming 650021, Yunnan Province, China
    5 Department of Pathology, Fourth Hospital of Kunming Medical University, Kunming 650021, Yunnan Province, China
    6 Institute of Neuroscience, Kunming Medical University, Kunming 650500, Yunnan Province, China
  • Received:2012-12-30 Revised:2013-04-07 Online:2013-06-05 Published:2013-06-05
  • Contact: Zhongtang Feng, Professor, Doctoral supervisor, Institute of Neuroscience, Kunming Medical University, Kunming 650500, Yunnan Province, China, zhongtangfeng@ sina.com.
  • About author:Yong Yin☆, Studying for doctorate, Chief physician.
  • Supported by:

    云南省科技厅-昆明医学院联合专项基金(2008CD037)

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摘要：

运动再学习疗法可明显改善缺血性脑血管病所致的各种功能障碍，但机制尚不清楚。胶质纤维酸性蛋白和神经丝蛋白的表达变化可体现受损神经元和星形胶质细胞损伤修复的状态，血管内皮生长因子和碱性成纤维细胞生长因子可反映受损脑组织血管新生状况。实验通过电凝法阻断恒河猴右侧大脑中动脉M1段制备脑缺血损伤模型，造模后第3天开始进行60 d的运动再学习疗法训练。免疫组织化学染色，SPECT检查结果表明，训练后脑缺血猴梗死侧组织胶质纤维酸性蛋白、神经丝蛋白、血管内皮生长因子及碱性成纤维细胞生长因子阳性表达细胞数量较对侧明显增多，脑缺血猴不同脑区的脑血流量有所改善，梗死侧大脑各脑区血流恢复显著。采用卒中临床等级评定量表评定训脑缺血猴的神经功能变化，发现运动再学习疗法训练不仅促进其运动功能的恢复，而且对实验猴的意识状态、自我照顾能力、平衡能力明显改善。说明运动再学习疗法可明显促进脑缺血恒河猴梗死侧周围区神经元再生修复、血管新生及神经功能恢复。

关键词: 神经再生, 脑损伤, 脑卒中, 运动再学习疗法, 恒河猴, 脑缺血, 动物模型, 神经功能, 神经因子, SPECT, 脑血流, 基金资助文章

Abstract:

The motor relearning program can significantly improve various functional disturbance induced by ischemic cerebrovascular diseases. However, its mechanism of action remains poorly understood. In injured brain tissues, glial fibrillary acidic protein and neurofilament protein changes can reflect the condition of injured neurons and astrocytes, while vascular endothelial growth factor and basic fibroblast growth factor changes can indicate angiogenesis. In the present study, we induced ischemic brain injury in the rhesus macaque by electrocoagulation of the M1 segment of the right middle cerebral artery. The motor relearning program was conducted for 60 days from the third day after model establishment. Immunohistochemistry and single-photon emission CT showed that the numbers of glial fibrillary acidic protein-, neurofilament protein-, vascular endothelial growth factor- and basic fibroblast growth factor-positive cells were significantly increased in the infarcted side compared with the contralateral hemisphere following the motor relearning program. Moreover, cerebral blood flow in the infarcted side was significantly improved. The clinical rating scale for stroke was used to assess neurological function changes in the rhesus macaque following the motor relearning program. Results showed that motor function was improved, and problems with consciousness, self-care ability and balance function were significantly ameliorated. These findings indicate that the motor relearning program significantly promoted neuronal regeneration, repair and angiogenesis in the surroundings of the infarcted hemisphere, and improve neurological function in the rhesus macaque following brain ischemia.

Key words: neural regeneration, brain injury, stroke, motor relearning program, rhesus macaque, brain ischemia, animal model, neurological function, neurotrophic factor, single-photon emission CT, cerebral blood flow, grants-supported paper, neuroregeneration