中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (27): 2501-2509.doi: 10.3969/j.issn.1673-5374.2013.27.001

• 原著:周围神经损伤修复保护与再生 •    下一篇

纳米纤维神经导管修复长段坐骨神经缺损的有效性

  

  • 收稿日期:2013-05-02 修回日期:2013-07-16 出版日期:2013-09-25 发布日期:2013-09-25
  • 基金资助:

    伊朗伊斯兰阿扎德大学基金项目（No. 73/442453）

Efficacy of nanofibrous conduits in repair of long- segment sciatic nerve defects

Esmaeil Biazar1, Saeed Heidari Keshel2, 3, Majid Pouya4   

  1. 1 Department of Biomaterial Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran

    2 Student Research Committee, Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    3 Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    4 Faculty of Medical Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
  • Received:2013-05-02 Revised:2013-07-16 Online:2013-09-25 Published:2013-09-25
  • Contact: Esmaeil Biazar, Ph.D., Department of Biomaterial Engineering, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran, kia_esm@yahoo.com.
  • Supported by:

    This work was supported by Tonekabon Branch, Islamic Azad University, Tonekabon, Iran, No. 73/442453.

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摘要：

作者前期研究已从组织形态学角度证实，纳米纤维聚羟基丁酸戊酸共聚酯（PHBV）神经导管可修复30mm的长段坐骨神经缺损，但修复后对大鼠行为学改变会有何影响呢？我们以纳米纤维聚羟基丁酸戊酸共聚酯（PHBV）神经导管和自体坐骨神经桥接大鼠30 mm长的坐骨神经缺损，其后4个月，每月应用趾外偏角、足距宽度分析、行走足迹分析、伸肌伸张反射评估、游泳试验、旷场试验、伤害感受功能分析对坐骨神经功能恢复情况进行评估。结果显示神经导管桥接的坐骨神经功能恢复与自体神经移植修复效果相近。说明纳米纤维聚羟基丁酸戊酸共聚酯（PHBV）神经导管在长段缺损坐骨神经缺损修复方面具有应用潜力。

关键词: 神经再生, 周围神经损伤, 坐骨神经, 神经导管, 纳米纤维聚羟基丁酸戊酸共聚酯, 行为学, 运动功能, 伤害感受, 基金资助文章

Abstract:

Our previous studies have histomorphologically confirmed that nanofibrous poly(3-hydroxybutyrate- co-3-hydroxyvalerate) conduit can be used to repair 30-mm-long sciatic nerve defects. However, the repair effects on rat behaviors remain poorly understood. In this study, we used nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) conduit and autologous sciatic nerve to bridge 30-mm-long rat sciatic nerve gaps. Within 4 months after surgery, rat sciatic nerve functional re-covery was evaluated per month by behavioral analyses, including toe out angle, toe spread anal-ysis, walking track analysis, extensor postural thrust, swimming test, open-field analysis and noci-ceptive function. Results showed that rat sciatic nerve functional recovery was similar after nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) conduit and autologous nerve grafting. These findings suggest that nanofibrous poly(3-hydroxybutyrate-co-3-hydroxyvalerate) conduit is suitable in use for repair of long-segment sciatic nerve defects.

Key words: neural regeneration, peripheral nerve injury, sciatic nerve, nerve conduit, poly(3-hydroxybutyrate- co-3-hydroxyvalerate, behaviors, motor function, nociceptive function, grants-supported paper, neuroregeneration