中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (30): 2838-2848.doi: 10.3969/j.issn.1673-5374.2013.30.006

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

丙戊酸干预臂丛神经撕脱伤后保护神经元并促进其再生

  

  • 收稿日期:2013-06-13 修回日期:2013-08-29 出版日期:2013-10-25 发布日期:2013-10-25
  • 基金资助:

    吉林大学研究生创新研究计划（20121115）；国家自然科学基金项目(No. 30872626)；卫生部临床学科重点项目(No.439)；高等学校博士学科点专项科研基金项目(No. 20070183143)

     

Valproic acid protects neurons and promotes neuronal regeneration after brachial plexus avulsion

Qiang Li1, Dianxiu Wu1, Rui Li1, Xiaojuan Zhu2, Shusen Cui1   

  1. 1 Department of Hand Surgery, China Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China
    2 Key Laboratory of Molecular Epigenetics of Ministry of Education, Institute of Cytology and Genetics, Northeast Normal University, Changchun 130000, Jilin Province, China
  • Received:2013-06-13 Revised:2013-08-29 Online:2013-10-25 Published:2013-10-25
  • Contact: Rui Li, M.D., Professor, Chief physician, Department of Hand Surgery, China Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China, 13304321102 @qq.com. Shusen Cui, Professor, Doctoral supervisor, Department of Hand Surgery, China Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China, sscui916@126.com.
  • About author:Qiang Li, M.D., Attending physician. Qiang Li and Dianxiu Wu contributed equally to this work.
  • Supported by:

    This work was supported by Graduated Innovation Fund of Jilin University, No. 20121115; the National Natural Science Foundation of China, No. 30872626; Key Projects of Clinical Sciences by the Ministry of Health, No. 439; and the Research Fund for the Doctoral Program of Higher Education, No. 20070183143

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摘要：

动物实验已经证明丙戊酸具有对周围神经的保护作用，并能促进轴突再生。但其对臂丛撕脱伤能否发挥同样的作用尚未见报道。实验建立大鼠臂丛根性撕脱伤模型，分别用含丙戊酸的饮用水（300mg/kg/d）和正常水喂饲。在伤后的1，2，3，7，14，28d采集损伤侧C5-T1脊髓节段标本，进行Real-time PCR和western blot检测Bcl-2, c-Jun和GAP-43的mRNA和蛋白表达。结果显示，丙戊酸使臂丛撕脱伤后，C5-T1脊髓节段中Bcl-2和GAP-43表达明显升高，c-jun表达明显降低。提示丙戊酸对臂丛撕脱伤后对应的脊髓神经元有保护和促进再生作用。

关键词: 神经再生, 周围神经损伤, 臂丛根性撕脱伤, 脊髓, 神经元, 丙戊酸, 神经保护, 神经元再生, Bcl-2, c-jun, GAP-43, NSFC

Abstract:

Valproic acid has been shown to exert neuroprotective effects and promote neurite outgrowth in several peripheral nerve injury models. However, whether valproic acid can exert its beneficial effect on neurons after brachial plexus avulsion injury is currently unknown. In this study, brachial plexus root avulsion models, established in Wistar rats, were administered daily with valproic acid dis-solved in drinking water (300 mg/kg) or normal water. On days 1, 2, 3, 7, 14 and 28 after avulsion injury, tissues of the C5–T1 spinal cord segments of the avulsion injured side were harvested to in-vestigate the expression of Bcl-2, c-Jun and growth associated protein 43 by real-time PCR and western blot assay. Results showed that valproic acid significantly increased the expression of Bcl-2 and growth associated protein 43, and reduced the c-Jun expression after brachial plexus avulsion. Our findings indicate that valproic acid can protect neurons in the spinal cord and enhance neuronal regeneration following brachial plexus root avulsion.

Key words: neural regeneration, peripheral nerve injury, brachial plexus root avulsion, spinal cord, neurons, valproic acid, neuroprotection, neuronal regeneration, Bcl-2, c-Jun, GAP-43, grants-supported pa-per, neuroregeneration