中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (2): 237-243.doi: 10.4103/1673-5374.152377

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

椎间盘脱出大鼠受压脊髓神经元形态和功能的回复：电针作用验证

  

  • 收稿日期:2014-10-22 出版日期:2015-02-17 发布日期:2015-02-17
  • 基金资助:

    中国国家自然科学基金项目（31372473，30871886），北京市属高校人才强教深化计划（PHR201107134），北京农学院科研项目(PXM2012_014207_000010)

Electroacupuncture improves microcirculation and neuronal morphology in the spinal cord of a rat model of intervertebral disc extrusion

Dai-xun Jiang 1, Zhi-song Lu 1, Ge-bin Li 1, 2, Sheng-yong Sun 1, Xiang Mu 1, Peter Lee 2, Wu Chen 1   

  1. 1 Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, China
    2 Department of Veterinary Biochemistry, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan
  • Received:2014-10-22 Online:2015-02-17 Published:2015-02-17
  • Contact: Wu Chen, Ph.D., tcvmchenwu@hotmail.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 31372473, 30871886; the Funding Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality of China, No. PHR201107134; the 2012 Scientific Research Quality Raising Funds of Beijing University of Agriculture of China, No. PXM2012_014207_000010.

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摘要：

受损脊髓神经元的研究中，大多侧重于脊髓组织形态和神经细胞损伤及修复相关基因的表达，而微循环在脊髓损伤和修复过程中的重要性常常被忽略。为此，实验以硅胶片填充于大鼠T13的脊髓腹侧位，制作出胸腰段椎间盘脱出的后肢瘫痪模型。电针刺激两侧足三里和内庭穴位14d后，模型大鼠L1段脊髓背侧血流量显著升高，T13段脊髓微血管密度显著升高，脊髓腹角中正常神经元数量增加，脊髓白质空泡化程度减轻，无明显神经胶质细胞增生现象，大鼠后肢运动功能显著改善。结果提示电针能改善椎间盘脱出模型大鼠脊髓神经元形态和微循环，促进神经功能的恢复。

关键词: 神经再生, 电针, 椎间盘, 血液循环, 炎症, 神经保护, 运动功能, 神经元

Abstract:

Most studies on spinal cord neuronal injury have focused on spinal cord tissue histology and the expression of nerve cell damage and repair-related genes. The importance of the microcirculation is often ignored in spinal cord injury and repair research. Therefore, in this study, we established a rat model of intervertebral disc extrusion by inserting a silica gel pad into the left ventral surface of T13. Electroacupuncture was used to stimulate the bilateral Zusanli point (ST36) and Neiting point (ST44) for 14 days. Compared with control animals, blood flow in the first lumbar vertebra (L1) was noticeably increased in rats given electroacupuncture. Microvessel density in the T13 segment of the spinal cord was increased significantly as well. The number of normal neurons was higher in the ventral horn of the spinal cord. In addition, vacuolation in the white matter was lessened. No obvious glial cell proliferation was visible. Furthermore, hindlimb motor function was improved significantly. Collectively, our results suggest that electroacupuncture can improve neuronal morphology and microcirculation, and promote the recovery of neurological functions in a rat model of intervertebral disc extrusion.

Key words: nerve regeneration, electroacupuncture, intervertebral disc, blood circulation, inflammation, neuroprotection, motor function, neurons, NSFC grants, neural regeneration