中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (5): 816-822.doi: 10.4103/1673-5374.182710

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

重复磁刺激对脊髓损伤的保护

  

  • 收稿日期:2016-01-26 出版日期:2016-05-20 发布日期:2016-05-20

Repetitive magnetic stimulation affects the microenvironment of nerve regeneration and evoked potentials after spinal cord injury

Jin-lan Jiang1, 2, #, Xu-dong Guo3, Shu-quan Zhang4, Xin-gang Wang5, Shi-feng Wu5, *, #   

  1. "1 Scientific Research Center, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China 2 Department of Orthopedics, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China 3 Department of Cardiovascular Medicine, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China 4 Department of Orthopedics, Tianjin Nankai Hospital, Tianjin, China 5 Department of Burns and Plastic Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China"
  • Received:2016-01-26 Online:2016-05-20 Published:2016-05-20
  • Contact: Shi-feng Wu, wsf19770620@126.com.

摘要:

研究发现重复磁刺激可以改变大脑局部的血流量,能兴奋皮质脊髓束以至肌肉的整个运动系统,达到促进运动功能恢复的目的。我们按照改良的Allen打击法建立大鼠急性脊髓损伤模型,损伤后4h开始经脊髓重复磁刺激,刺激强度为最大输出强度的35%,刺激频率为5Hz,每序列5 s,间歇2 min,连续10个序列,1次/d,每周5 d,连续2周。经脊髓重复磁刺激后,脊髓损伤大鼠脊髓组织中细胞凋亡细胞数量减少,基质金属蛋白酶9/2基因和蛋白表达降低,巢蛋白表达升高,体感诱发电位和运动诱发电位恢复以及运动能力恢复。结果提示脊髓重复磁刺激改善了脊髓神经再生微环境,减少脊髓损伤区神经细胞的凋亡,对损伤脊髓起到神经保护和修复作用。 

orcid: 0000-0002-7326-3018(Shi-feng Wu)

关键词: 脊髓损伤, 经脊髓重复磁刺激, 神经再生, 运动功能, 大鼠, 康复, 可塑性, 再生微环境

Abstract:

"Repetitive magnetic stimulation has been shown to alter local blood flow of the brain, excite the corticospinal tract and muscle, and induce motor function recovery. We established a rat model of acute spinal cord injury using the modified Allen’s method. After 4 hours of injury, rat models received repetitive magnetic stimulation, with a stimulus intensity of 35% maximum output intensity, 5-Hz frequency, 5 seconds for each sequence, and an interval of 2 minutes. This was repeated for a total of 10 sequences, once a day, 5 days in a week, for 2 consecutive weeks. After repetitive magnetic stimulation, the number of apoptotic cells decreased, matrix metalloproteinase 9/2 gene and protein expression decreased, nestin expression increased, somatosensory and motor-evoked potentials recovered, and motor function recovered in the injured spinal cord. These findings confirm that repetitive magnetic stimulation of the spinal cord improved the microenvironment of neural regeneration, reduced neuronal apoptosis, and induced neuroprotective and repair effects on the injured spinal cord."

Key words: "nerve regeneration, spinal cord injury, repetitive magnetic stimulation, motor function, rats, rehabilitation, plasticity, regenerative microenvironment, neural regeneration"