中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2024, Vol. 19 ›› Issue (9): 1998-2003.doi: 10.4103/1673-5374.390959

• 综述:脑损伤修复保护与再生 • 上一篇    下一篇

干细胞治疗缺血性脑卒中多层组学研究的优势

  

  • 出版日期:2024-09-15 发布日期:2024-01-25
  • 基金资助:
    国家重点研发计划项目（2018YFA0108602）；CAMS创新医学项目（2021-1-I2M-019）；国家高水平医院临床研究基金项目（2022-PUMCH-C-042）

The advantages of multi-level omics research on stem cell-based therapies for ischemic stroke

Yiqing Wang1, 2, Chuheng Chang1, 2, Renzhi Wang2, *, Xiaoguang Li3, 4, *, Xinjie Bao2, *   

  1. 14+4 Doctor Medical Program, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China; 2Department of Neurosurgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, China; 3Beijing Key Laboratory for Biomaterials and Neural Regeneration, School of Biological Science and Medical Engineering, Beihang University, Beijing, China; 4Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing, China
  • Online:2024-09-15 Published:2024-01-25
  • Contact: Xinjie Bao, MD, Baoxinjie1@pumch.cn; Xiaoguang Li, PhD, lxgchina@sina.com; Renzhi Wang, MD, wangrz@126.com.
  • Supported by:
    This work was supported by the National Key Research and Development Program of China, No. 2018YFA0108602; the CAMS Initiative for Innovative Medicine, No. 2021-1-I2M-019; the National High Level Hospital Clinical Research Funding, No. 2022-PUMCH-C-042 (all to XB).

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摘要：

干细胞移植疗法是治疗缺血性脑卒中的潜在方法之一。尽管已进行多年的临床前研究，但干细胞在临床环境中的应用仍在探索中。尽管干细胞在促进缺血性脑卒中功能恢复方面具有显著价值，但对其确切作用机制仍未完全探明。组学为了解缺血性脑卒中中干细胞疗法作用机制提供了一个新的平台。转录组、蛋白质组和代谢组已成为发现生物标志物和分析病理条件下分子变化的重要方法。组学分析有助于识别干细胞治疗的缺血性脑卒中中干细胞介导的新通路，从而促进干细胞疗法的临床转化。因此，此次综述总结了多组学技术在分析干细胞治疗缺血性脑卒中中的病理生理学和最新研究进展，并讨论干细胞治疗对缺血性脑卒中大脑组织和体液的RNA、蛋白质和代谢产物的影响，还归纳了对细胞疗法起关键作用的调控因子。文章对于干细胞疗法分子水平的探索有助于推动干细胞向临床转化，为缺血性脑卒中患者神经功能的完全恢复提供新的干预可能。

https://orcid.org/0000-0003-2117-7692 (Xinjie Bao); https://orcid.org/0000-0003-4480-3676 (Xiaoguang Li); https://orcid.org/0000-0003-2080-5474 (Renzhi Wang)

关键词: 缺血性脑卒中, 多组学, 病理生理学, 神经炎症, 干细胞疗法, 间充质干细胞, 神经干/祖细胞, 转录组学, 蛋白质组学, 代谢组学

Abstract: Stem cell transplantation is a potential therapeutic strategy for ischemic stroke. However, despite many years of preclinical research, the application of stem cells is still limited to the clinical trial stage. Although stem cell therapy can be highly beneficial in promoting functional recovery, the precise mechanisms of action that are responsible for this effect have yet to be fully elucidated. Omics analysis provides us with a new perspective to investigate the physiological mechanisms and multiple functions of stem cells in ischemic stroke. Transcriptomic, proteomic, and metabolomic analyses have become important tools for discovering biomarkers and analyzing molecular changes under pathological conditions. Omics analysis could help us to identify new pathways mediated by stem cells for the treatment of ischemic stroke via stem cell therapy, thereby facilitating the translation of stem cell therapies into clinical use. In this review, we summarize the pathophysiology of ischemic stroke and discuss recent progress in the development of stem cell therapies for the treatment of ischemic stroke by applying multi-level omics. We also discuss changes in RNAs, proteins, and metabolites in the cerebral tissues and body fluids under stroke conditions and following stem cell treatment, and summarize the regulatory factors that play a key role in stem cell therapy. The exploration of stem cell therapy at the molecular level will facilitate the clinical application of stem cells and provide new treatment possibilities for the complete recovery of neurological function in patients with ischemic stroke.

Key words: ischemic stroke, mesenchymal stem cells, metabolomics, multilevel omics, neural stem/progenitor cells, neuroinflammation, pathophysiology, proteomics, stem cell therapy, transcriptomes