中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (10): 1669-1673.doi: 10.4103/1673-5374.167768

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

不同损伤机制下股神经的再生及其精确性

  

  • 收稿日期:2015-08-21 出版日期:2015-10-28 发布日期:2015-10-28
  • 基金资助:

    国家自然科学基金 (81360194)

Femoral nerve regeneration and its accuracy under different injury mechanisms

Aikeremujiang·Muheremu1, 2, Qiang Ao3, Yu Wang2, Peng Cao4, Jiang Peng2, *   

  1. 1 Medical Center, Tsinghua University, Beijing, China
    2 Institute of Orthopaedics, General Hospital of People’s Liberation Army, Beijing, China
    3 Department of Tissue Engineering, China Medical University, Shenyang, Liaoning, China
    4 Department of Orthopedics, Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, China
  • Received:2015-08-21 Online:2015-10-28 Published:2015-10-28
  • Contact: Jiang Peng, M.D., Ph.D.,pengjdxx@126.com.
  • Supported by:

    This research was supported by the National Natural Science Foundation of China, No. 81360194, and a grant from the National Basic Research Program of China, No. 2014CB542200.

摘要:

虽然显微缝合技术能实现周围神经断端之间的精确吻合,但由于再生神经轴突无法精确长入到远端神经通道内,损伤神经靶器官功能恢复不能完全实现。已有很多的研究试图探索再生神经轴突错向再生的机制,但尚无统一的观点。为此,我们的实验拟揭示周围神经损伤后神经选择性再生的机制,在大鼠右侧股神经利用钳夹损伤以及冰冻损伤后2,3,6,12周,利用逆向示踪方法评价神经再生的准确性及其变化趋势,钳夹损伤组与冰冻损伤组大鼠脊髓前角神经元中用DIL红色染神经元的数量和比例逐渐随损伤后时间延长而增高。损伤后各时间点钳夹损伤组和冰冻损伤组大鼠脊髓前角神经元数量和比例之间未见明显差异。结果证实大鼠股神经钳夹损伤以及冰冻损伤后神经轴突再生的准确性随在损伤后2,3,6,12周逐渐提高,冰冻损伤的大鼠股神经虽然有较长阶段的神经损伤,但仍然能达到较精确的轴突再生。

关键词: 神经再生, 周围神经损伤, 周围神经, 再生, 机制, 趋化性, 趋向性再生, 动物实验, 逆行示踪, 选择性神经再生, 功能恢复, 动物模型, 国家自然科学基金

Abstract:

Surgical accuracy has greatly improved with the advent of microsurgical techniques. However, complete functional recovery after peripheral nerve injury has not been achieved to date. The mechanisms hindering accurate regeneration of damaged axons after peripheral nerve injury are in urgent need of exploration. The present study was designed to explore the mechanisms of peripheral
nerve regeneration after different types of injury. Femoral nerves of rats were injured by crushing or freezing. At 2, 3, 6, and 12 weeks after injury, axons were retrogradely labeled using 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (Dil) and True Blue, and motor and sensory axons that had regenerated at the site of injury were counted. The number and percentage of Dil-labeled neurons in the anterior horn of the spinal cord increased over time. No significant differences were found in the number of labeled neurons between the freeze and crush injury groups at any time point. Our results confirmed that the accuracy of peripheral nerve regeneration increased with time, after both crush and freeze injury, and indicated that axonal regeneration accuracy was still satisfactory after freezing, despite the prolonged damage.