中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (8): 1638-1644.doi: 10.4103/1673-5374.301023

• 原著:退行性病与再生 • 上一篇    下一篇

嗅鞘细胞移植调节脊髓硫酸软骨素蛋白聚糖表达促进脊髓损伤后的轴突再生

  

  • 出版日期:2021-08-15 发布日期:2021-01-13
  • 基金资助:

    陕西省2018年重点研究发展计划（2018SF-124）；国家重点研究发展项目（2018YFE0114200）

Olfactory ensheathing cell transplantation alters the expression of chondroitin sulfate proteoglycans and promotes axonal regeneration after spinal cord injury

Guo-Yu Wang1, Zhi-Jian Cheng1, Pu-Wei Yuan1, Hao-Peng Li1, Xi-Jing He1, 2, *   

  1. 1Second Department of Orthopedics, the Second Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi Province, China; 2Xi’an International Medical Center Orthopedic Hospital, Xi’an, Shaanxi Province, China
  • Online:2021-08-15 Published:2021-01-13
  • Contact: Xi-Jing He, MD, xijing_h@vip.tom.com.
  • Supported by:
    This study was supported by Shaanxi Provincial Key Research and Development Plan in 2018, No. 2018SF-124 (to GYW) and National Key Research and Development Project of the People’s Republic of China, No. 2018YFE0114200 (to XJH).

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摘要：

细胞移植是一种潜在的脊髓损伤治疗方法，其中嗅鞘细胞由于具有星形胶质细胞和许旺细胞的双重特征，在移植修复脊髓损伤中有积极作用，但其具体的作用机制尚待研究。实验通过横断T10建立脊髓损伤大鼠模型，在脊髓断端1 mm处注射嗅鞘细胞进行治疗。结果发现：（1）嗅鞘细胞移植可以在一定程度上恢复脊髓损伤大鼠的后肢运动功能，但对神经胶质瘢痕的形成和体积没有影响，同时减少损伤部位硫酸软骨素蛋白聚糖（NG2和Neurocan）以及神经胶质原纤维酸性蛋白免疫阳性反应，增加生长相关蛋白43和神经丝的免疫阳性反应；（2）说明嗅鞘细胞移植可通过调节脊髓硫酸软骨素蛋白聚糖表达，抑制神经胶质细胞过度增殖致瘢痕形成，增加再生神经纤维数量，进而促进脊髓损伤后的轴突再生。实验于2018年9月9日经西安交通大学医学院动物伦理委员会批准，批准号2018-2048。

https://orcid.org/0000-0001-6049-2551 (Guo-Yu Wang)

关键词: 中枢神经系统, 脊髓, 模型, 大鼠, 体内, 损伤, 修复, 保护, 再生, 细胞, 神经功能

Abstract: Cell transplantation is a potential treatment for spinal cord injury. Olfactory ensheathing cells (OECs) play an active role in the repair of spinal cord injury as a result of the dual characteristics of astrocytes and Schwann cells. However, the specific mechanisms of repair remain poorly understood. In the present study, a rat model of spinal cord injury was established by transection of T10. OECs were injected into the site, 1 mm from the spinal cord stump. To a certain extent, OEC transplantation restored locomotor function in the hindlimbs of rats with spinal cord injury, but had no effect on the formation or volume of glial scars. In addition, OEC transplantation reduced the immunopositivity of chondroitin sulfate proteoglycans (neural/glial antigen 2 and neurocan) and glial fibrillary acidic protein at the injury site, and increased the immunopositivity of growth-associated protein 43 and neurofilament. These findings suggest that OEC transplantation can regulate the expression of chondroitin sulfate proteoglycans in the spinal cord, inhibit scar formation caused by the excessive proliferation of glial cells, and increase the numbers of regenerated nerve fibers, thus promoting axonal regeneration after spinal cord injury. The study was approved by the Animal Ethics Committee of the Medical College of Xi’an Jiaotong University, China (approval No. 2018-2048) on September 9, 2018.

Key words: cells, central nervous system, in vivo, injury, model, neurological function protection, rat, regeneration, repair, spinal cord