中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2021, Vol. 21 ›› Issue (5): 2040-2049.doi: 10.4103/NRR.NRR-D-24-00995

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

利用霍乱毒素B亚基追踪恒河猴脊髓运动神经元和初级感觉传入神经

  

  • 出版日期:2026-05-15 发布日期:2025-08-23
  • 基金资助:
    这项研究得到了国家科技部重大项目（2022ZD0204704），国家自然科学基金青年项目（82301572）和中国博士后科学基金面上项目（2023M731202）的支持。

Tracing motor neurons and primary sensory afferents of the monkey spinal cord with cholera toxin subunit B

Ziyu He1, 2, #, Zhixian Liu1, 2, #, Wenjie Xu1, 2, Ruoying Zhang1, 2, Shu Fan1, 2, Wei Wang1, 2, 3, *, Xiaolong Zheng1, 2, *   

  1. 1 Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China;  2 Hubei Key Laboratory of Neural Injury and Functional Reconstruction, Huazhong University of Science and Technology, Wuhan, Hubei Province, China;  3 Key Laboratory of Neurological Diseases of Chinese Ministry of Education, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China
  • Online:2026-05-15 Published:2025-08-23
  • Contact: Wei Wang, PhD, MD, wwang@tjh.tjmu.edu.cn; Xiaolong Zheng, PhD, xl_zheng@hust.edu.cn.
  • Supported by:
    This work was supported by a grant from Ministry of Science and Technology China, No. 2022ZD0204704 (to WW); the National Natural Science Foundation of China, No. 82301572 (to XZ); and the China Postdoctoral Science Foundation, No. 2023M731202 (to XZ).

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摘要：

非人灵长类动物正日益成为神经科学研究的对象。然而，目前还缺乏对猴脊髓运动神经元和初级感觉传入神经进行标记的有效神经元追踪技术。实验通过给予恒河猴坐骨神经注射霍乱毒素 B 亚基，成功地标记了腰段和骶段脊髓中的运动神经元和初级感觉传入神经纤维。标记的α运动神经元位于 L6-S1 节段的第9层，支配屈肌和伸肌。被标记的初级感觉传入神经纤维主要是髓鞘化的 Aβ 纤维，主要终止于腰4-7 节段的 I-II 层；这些传入神经与被标记的本体感觉传入神经纤维一起与多种类型的脊髓神经元形成兴奋性突触。总之，实验方法可为恒河猴脊髓的神经元连接提供解剖学证据，并可用于非人灵长类动物的脊髓研究。结果表明，霍乱毒素B亚基能够有效标记恒河猴脊髓中的运动神经元和感觉传入神经，为神经示踪技术提供了一种稳定有效的方法。这一发现为进一步探索NHPs中神经元连接和功能重建提供了重要工具。

https://orcid.org/0000-0002-9176-5150 (Wei Wang); https://orcid.org/0000-0002-4164-0499 (Xiaolong Zheng)

关键词: 霍乱毒素亚单位 B, 中间神经元, 猕猴, 运动神经元, 神经元追踪, 初级感觉传入, 猕猴, 坐骨神经, 脊髓

Abstract: Nonhuman primates are increasingly being used as animal models in neuroscience research. However, efficient neuronal tracing techniques for labeling motor neurons and primary sensory afferents in the monkey spinal cord are lacking. Here, by injecting the cholera toxin B subunit into the sciatic nerve of a rhesus monkey, we successfully labeled the motor neurons and primary sensory afferents in the lumbar and sacralspinal cord. Labeled alpha motor neurons were located in lamina IX of the L6–S1 segments, which innervate both flexors and extensors. The labeled primary sensory afferents were mainly myelinated Aβ fibers that terminated mostly in laminae I and II of the L4–L7 segments. Together with the labeled proprioceptive afferents, the primary sensory afferents formed excitatory synapses with multiple types of spinal neurons. In summary, our methods successfully traced neuronal connections in the monkey spinal cord and can be used in spinal cord studies when nonhuman primates are used.

Key words: cholera toxin subunit B, interneuron, Macaca Mulatta, monkey, motor neuron, neuron tracing, primary sensory afferents, rhesus macaque, sciatic nerve, spinal cord