中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (8): 1305-1311.doi: 10.4103/1673-5374.162765

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

丙泊酚增强骨髓间充质干细胞移植修复脊髓损伤的效果

  

  • 收稿日期:2015-06-03 出版日期:2015-08-24 发布日期:2015-08-24

Propofol promotes spinal cord injury repair by bone marrow mesenchymal stem cell transplantation

Ya-jing Zhou1, Jian-min Liu2, Shu-ming Wei3, Yun-hao Zhang1, Zhen-hua Qu1, Shu-bo Chen4   

  1. 1 Department of Anesthesiology, Xingtai People’s Hospital, Hebei Medical University, Xingtai, Hebei Province, China
    2 Department of Orthopedic Trauma, Xingtai People’s Hospital, Hebei Medical University, Xingtai, Hebei Province, China
    3 Department of Anesthesiology, Third Hospital of Hebei Medical University, Shijiazhuang, Hebei Province, China
    4 Department of Urinary Surgery, Xingtai People’s Hospital, Hebei Medical University, Xingtai, Hebei Province, China
  • Received:2015-06-03 Online:2015-08-24 Published:2015-08-24
  • Contact: Shu-bo Chen, baidong_2@163.com.

摘要:

丙泊酚是具有神经保护作用的麻醉药物,将其与骨髓间充质干细胞移植联合应用,是否可增强其修复损伤神经的作用?我们将骨髓间充质干细胞移植与丙泊酚联合,观察丙泊酚尾静脉泵注对骨髓间充质干细胞移植修复大鼠脊髓损伤的影响。结果显示,两者联合治疗脊髓损伤大鼠的脊髓病理损伤明显减轻,脊髓组织中可见大量新生的无髓及有髓神经纤维,CM-DIL标记骨髓间充质干细胞及荧光金标记的阳性神经纤维数量增加,脊髓损伤大鼠后肢运动功能明显改善,且效果优于单独骨髓间充质干细胞移植或丙泊酚治疗。说明丙泊酚联合骨髓间充质干细胞移植修复大鼠脊髓损伤能提高其治疗的效果。

关键词: 神经再生, 骨髓间充质干细胞, 干细胞移植, 丙泊酚, 大鼠, 脊髓损伤, 修复, 神经保护, 麻醉

Abstract:

Propofol is a neuroprotective anesthetic. Whether propofol can promote spinal cord injury repair by bone marrow mesenchymal stem cells remains poorly understood. We used rats to investigate spinal cord injury repair using bone marrow mesenchymal stem cell transplantation combined with propofol administration via the tail vein. Rat spinal cord injury was clearly alleviated; a large number of newborn non-myelinated and myelinated nerve fibers appeared in the spinal cord, the numbers of CM-Dil-labeled bone marrow mesenchymal stem cells and fluorogold-labeled nerve fibers were increased and hindlimb motor function of spinal cord-injured rats was markedly improved. These improvements were more prominent in rats subjected to bone marrow mesenchymal cell transplantation combined with propofol administration than in rats receiving monotherapy. These results indicate that propofol can enhance the therapeutic effects of bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

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