中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (5): 836-841.doi: 10.4103/1673-5374.297086

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

D-丝氨酸可减轻慢性铅暴露所致神经元损伤和记忆障碍

  

  • 出版日期:2021-05-15 发布日期:2020-12-29
  • 基金资助:

    国家自然科学基金项目(8167320831802194

D-serine reduces memory impairment and neuronal damage induced by chronic lead exposure

Jian-Zhu Bo1, #, Ling Xue1, #, Shuang Li2, Jing-Wen Yin1, Zheng-Yao Li1, Xi Wang1, Jun-Feng Wang1, Yan-Shu Zhang1, 2, *#br#   

  1. 1 College of Public Health, North China University of Science and Technology, Tangshan, Hebei Province, China;  2 Laboratory Animal Center, North China University of Science and Technology, Tangshan, Hebei Province, China
  • Online:2021-05-15 Published:2020-12-29
  • Contact: Yan-Shu Zhang, PhD, yangshu_zhang@163.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 81673208 (to YSZ), and 31802194 (to SL).

摘要:

尽管已证明外源性D-丝氨酸是一种神经调节剂,但其在铅暴露引起的海马损伤中的作用尚不清楚。实验以0.05%乙酸铅作为大鼠饮用水,连续干预8周建立慢性铅暴露模型,同时每天2次腹腔注射3060 mg/kg D-丝氨酸。见D-丝氨酸在一定程度上缩短了铅暴露引起的大鼠水迷宫逃避潜伏期,增加了穿越目标平台的次数,并减轻了海马神经元病理损伤。尽管D-丝氨酸不能改善铅暴露大鼠海马中N-甲基-D-天冬氨酸受体NR2B的表达,但60mg/kg D-丝氨酸可恢复铅暴露引起的海马NR2A的低表达。表明D-丝氨酸可改善铅暴露引起的学习和记忆能力损伤,且其机制与增加海马NR2A表达有关。实验于20181221日经华北科技大学实验动物伦理委员会批准,批准号LX2018155

https://orcid.org/0000-0003-1207-7251 (Yan-Shu Zhang)

关键词: 铅, 海马, D-丝氨酸, N-甲基-D-天冬氨酸, 中毒, 神经功能, 修复, 保护

Abstract: Although exogenous D-serine has been applied as a neural regulatory intervention in many studies, the role played by D-serine in hippocampal injuries caused by lead exposure remains poorly understood. Rat models of chronic lead exposure were established through the administration of 0.05% lead acetate for 8 weeks. Simultaneously, rats were administered 30 or 60 mg/kg D-serine, intraperitoneally, twice a day. Our results showed that D-serine treatment shortened the escape latency from the Morris water maze, increased the number of times that mice crossed the original platform location, and alleviated the pathological damage experienced by hippocampal neurons in response to lead exposure. Although D-serine administration did not increase the expression levels of the N-methyl-D-aspartate receptor subtype 2B (NR2B) in the hippocampi of lead-exposed rats, 60 mg/kg D-serine treatment restored the expression levels of NR2A, which are reduced by lead exposure. These findings suggested that D-serine can alleviate learning and memory impairments induced by lead exposure and that the underlying mechanism is associated with the increased expression of NR2A in the hippocampus. This study was approved by the Animal Ethics Committee of North China University of Science and Technology, China (approval No. LX2018155) on December 21, 2018. 

Key words: D-serine, hippocampus, lead, neurological function, N-methyl-D-aspartate, poisoning, protection, repair