中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (6): 589-593.doi: 10.4103/1673-5374.130097

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

葛根素能有效促进损伤坐骨神经的修复

  

  • 收稿日期:2014-02-26 出版日期:2014-03-22 发布日期:2014-03-22

Puerarin accelerates neural regeneration after sciatic nerve injury

Minfei Wu 1, Guanjie Zhao 2, Xiaoyu Yang 1, Chuangang Peng 1, Jianwu Zhao 1, Jun Liu 1, Rui Li 3, Zhongli Gao 1   

  1. 1 Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China
    2 Department of Nephropathy, China-Japan Union Hospital of Jilin University, Changchun, Jilin Province, China
    3 Hand & Foot Surgery and Reparative & Reconstruction Surgery Center, the Second Hospital of Jilin University, Changchun, Jilin Province, China
  • Received:2014-02-26 Online:2014-03-22 Published:2014-03-22
  • Contact: Zhongli Gao, Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin Province, China, zhongligao@sina.com. Rui Li, Hand & Foot Surgery and Reparative & Reconstruction Surgery Center, the Second Hospital of Jilin University, Changchun 130033, Jilin Province, China, 13304321102@qq.com.

摘要:

根据传统中药野葛提取物葛根素对脑缺血的保护作用,作者提出葛根素也有修复损伤周围神经的作用。实验采用10,5,2.5 mg/(kg.d)剂量的葛根素(4,7-二羟基8-β-D吡喃葡萄糖醛基异黄酮)注射坐骨神经损伤模型小鼠腹腔。建模后1,2,4周,高、中剂量葛根素显著上调了模型小鼠L4-6脊髓节段内生长相关蛋白43 mRNA和蛋白的表达;建模后8周,高、中剂量葛根素明显改善坐骨神经损伤模型小鼠损伤侧小腿三头肌的萎缩现象并促进了损伤侧脊髓神经纤维的再生。结果证实,葛根素持续激活上调坐骨神经脊髓相应节段细胞中生长相关蛋白43的表达,发回了促进损伤坐骨神经修复的效应。

关键词: 神经再生, 周围神经损伤, 葛根素, 周围神经损伤, 生长相关蛋白43, 坐骨神经, 修复, 国家自然科学基金

Abstract:

Puerarin is a natural isoflavone isolated from plants of the genus Pueraria and functions as a protector against cerebral ischemia. We hypothesized that puerarin can be involved in the repair of peripheral nerve injuries. To test this hypothesis, doses of 10, 5, or 2.5 mg/kg per day puerarin (8-(β-D-Glucopyranosyl-7-hydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one) were injected intraperitoneally into mouse models of sciatic nerve injury. Puerarin at the middle and high doses significantly up-regulated the expression of growth-associated protein 43 in the L4–6 segments of the spinal cord from mice at 1, 2, and 4 weeks after modeling, and reduced the atrophy of the triceps surae on the affected side and promoted the regeneration of nerve fibers of the damaged spinal cord at 8 weeks after injury. We conclude that puerarin exerts an ongoing role to activate growth-associated protein 43 in the corresponding segment of the spinal cord after sciatic nerve injury, thus contributing to neural regeneration after sciatic nerve injuries.