中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (20): 1820-1823.doi: 10.4103/1673-5374.143429

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

冷冻处理同种异体周围神经桥接缺损坐骨神经：神经节苷脂促进神经再生

  

  • 收稿日期:2014-08-20 出版日期:2014-10-25 发布日期:2014-10-25

Ganglioside promotes the bridging of sciatic nerve defects in cryopreserved peripheral nerve allografts

Yaodong Wang 1, Yuguang Liu 1, Qiang Liu 1, 2, 3   

  1. 1 Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong Province, China
    2 Brain Science Research Institute, Shandong University, Jinan, Shandong Province, China
    3 Department of Neurology, Third Affiliated Hospital of Liaoning Medical University, Jinzhou, Liaoning Province, China
  • Received:2014-08-20 Online:2014-10-25 Published:2014-10-25
  • Contact: Yuguang Liu, Ph.D., Brain Science Research Institute, Shandong University, Jinan City Cultural Road No. 107, Jinan 250012, Shandong Province, China, wangyaodong741@126.com.

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摘要：

既往研究发现外源性神经节苷脂可以促进神经系统的再生和突触的形成。实验取新西兰家兔双侧坐骨神经制成长为10mm的神经段深低温冷冻储存后，将其复温用于异体桥接修复同种异体兔左侧坐骨神经10mm缺损，3d后于兔右侧后腿皮下注射1mL神经节苷脂溶液（1g/L）。与未注射神经节苷脂的大鼠相比，造模后2-12周，注射的大鼠在损伤后肌肉湿重比增加，造模后4-12周坐骨神经再生神经纤维有髓纤维数量、髓鞘厚度、纤维直径增加，8和12周坐骨神经电位波幅和传导速度上升，12周时传导潜伏期下降。结果显示神经节苷脂可促进冷冻处理同种异体周围神经移植修复缺损坐骨神经的神经再生。

关键词: 神经再生, 神经节苷脂, 周围神经, 桥接, 修复, 神经移植, 冷冻处理, 异体神经移植, 坐骨神经

Abstract:

Previous studies have shown that exogenous gangliosides promote nervous system regeneration and synapse formation. In this study, 10 mm sciatic nerve segments from New Zealand rabbits were thawed from cryopreservation and were used for the repair of left sciatic nerve defects through allograft bridging. Three days later, 1 mL ganglioside solution (1 g/L) was subcutaneously injected into the right hind leg of rabbits. Compared with non-injected rats, muscle wet weight ratio was increased at 2–12 weeks after modeling. The quantity of myelinated fibers in regenerated sciatic nerve, myelin thickness and fiber diameter were elevated at 4–12 weeks after modeling. Sciatic nerve potential amplitude and conduction velocity were raised at 8 and 12 weeks, while conduction latencies were decreased at 12 weeks. Experimental findings indicate that ganglioside can promote the regeneration of sciatic nerve defects after repair with cryopreserved peripheral nerve allografts.

Key words: nerve regeneration, ganglioside, peripheral nerve, bridge, repair, nerve graft, cryopreservation, nerve allograft, sciatic nerve, neural regeneration