中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (7): 1139-1146.doi: 10.4103/1673-5374.160111

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

单侧腰段脊髓损伤后再生困难原因与PirB在神经系统中的分布有关

  

  • 收稿日期:2015-06-10 出版日期:2015-07-24 发布日期:2015-07-24

Distribution of paired immunoglobulin-like receptor B in the nervous system related to regeneration difficulties after unilateral lumbar spinal cord injury

Wan-shu Peng, Chao Qi, Hong Zhang, Mei-ling Gao, Hong Wang, Fei Ren, Xia-qing Li   

  1. Department of Pathophysiology, Shanxi Medical University, Taiyuan, Shanxi Province, China
  • Received:2015-06-10 Online:2015-07-24 Published:2015-07-24
  • Contact: Xia-qing Li, Ph.D., xqli2013@126.com
  • Supported by:

    This project was supported by the National Natural Science Foundation of China, No. 81171178; the Natural Science Foundation of Shanxi Province in China, No. 2012011036-3; the Research Project of Shanxi Scholarship Council of China, No. 2012-047.

摘要:

PirB是中枢神经轴突再生及突触可塑性修复的一种重要髓磷脂抑制因子的功能性受体,参与神经再生的抑制,PirB对损伤神经修复的调节作用正逐渐引起人们的注意。为了解脊髓神经损伤后再生不能的原因,我们采用免疫荧光染色方法检测了单侧腰椎脊髓损伤10d时PirB在中枢神经和周围神经的分布情况。发现损伤10d时,患侧背根神经节、坐骨神经及脊髓节段PirB表达增加。背根神经节和坐骨神经中,PirB主要沿神经元和轴突膜分布。PirB呈弥漫性分布在脊髓背侧和腹侧区域。部分大脑双侧中央前回神经元PirB表达增强。表明脊髓损伤后10d损伤部位及脑和周围神经系统中与损伤部位有生理功能联系的部位中PirB表达的变化,说明PirB可能通过多个方位抑制损伤后的修复过程。

关键词: 神经再生, PirB, 髓磷脂抑制因子, 脊髓损伤, 外周神经系统, 中枢神经系统, 大脑皮质, 背根神经节

Abstract:

Paired immunoglobulin-like receptor B (PirB) is a functional receptor of myelin-associated inhibitors for axonal regeneration and synaptic plasticity in the central nervous system, and thus suppresses nerve regeneration. The regulatory effect of PirB on injured nerves has received a lot of attention. To better understand nerve regeneration inability after spinal cord injury, this study aimed to investigate the distribution of PirB (via immunofluorescence) in the central nervous system and peripheral nervous system 10 days after injury. Immunoreactivity for PirB increased in the dorsal root ganglia, sciatic nerves, and spinal cord segments. In the dorsal root ganglia and sciatic nerves, PirB was mainly distributed along neuronal and axonal membranes. PirB was found to exhibit a diffuse, intricate distribution in the dorsal and ventral regions. Immunoreactivity for PirB was enhanced in some cortical neurons located in the bilateral precentral gyri. Overall, the findings suggest a pattern of PirB immunoreactivity in the nervous system after unilateral spinal transection injury, and also indicate that PirB may suppress repair after injury.

Key words: nerve regeneration, paired immunoglobulin-like receptor B, myelin inhibitory factor, spinal cord injury, peripheral nervous system, central nervous system, cerebral cortex, dorsal root ganglion, neural regeneration