中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (8): 1598-1605.doi: 10.4103/1673-5374.294565

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

ISP和PAP4有助于恢复周围神经损伤后运动功能的恢复

  

  • 出版日期:2021-08-15 发布日期:2021-01-13
  • 基金资助:

    国家自然科学基金面上项目(81971165);国家基础研究计划(973计划)(2014CB542205)

ISP and PAP4 peptides promote motor functional recovery after peripheral nerve injury

Shi-Qin Lv1, Wutian Wu1, 2, *   

  1. 1Guangdong-Hong Kong-Macau Institute of CNS Regeneration, Ministry of Education CNS Regeneration Collaborative Joint Laboratory, Jinan University, Guangzhou, Guangdong Province, China; 2Re-Stem Biotechnology Co., Ltd., Suzhou, Jiangsu Province, China
  • Online:2021-08-15 Published:2021-01-13
  • Contact: Wutian Wu, PhD, wtwu@hku.hk.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 81971165; and the National Basic Research Program of China (973 Program), No. 2014CB542205 (both to WW).

摘要:

PAP4和ISP两种小分子多肽均能促进脊髓损伤动物的神经再生以及运动功能恢复,但两者在周围神经损伤中的作用尚不清楚。实验以C6腹侧神经根挤压建立臂丛神经损伤模型,连续21d行在损伤部位附近皮下注射PAP4(497µg/d,2次/d)或ISP(11µg/d,1次/d)进行治疗。结果发现,经ISP和PAP治疗后,臂丛神经损伤大鼠运动神经元的存活率提高,再生轴突以及神经肌肉接头数量增加,肌肉萎缩明显减轻,运动单位电反应明显增强,损伤上肢运动功能显著恢复。表明ISP和PAP4可促进周围神经损伤后运动功能的恢复。实验于2011年经暨南大学实验动物伦理委员会批准,批准号20111008001。

https://orcid.org/0000-0002-3662-9922 (Shi-Qin Lv)

关键词: 周围神经, 臂丛神经损伤, 挤压伤, ISP, PAP4, 运动功能, 轴突, 修复, 保护, 再生

Abstract: Both intracellular sigma peptide (ISP) and phosphatase and tensin homolog agonist protein (PAP4) promote nerve regeneration and motor functional recovery after spinal cord injury. However, the role of these two small peptides in peripheral nerve injury remains unclear. A rat model of brachial plexus injury was established by crush of the C6 ventral root. The rats were then treated with subcutaneous injection of PAP4 (497 µg/d, twice per day) or ISP (11 µg/d, once per day) near the injury site for 21 successive days. After ISP and PAP treatment, the survival of motoneurons was increased, the number of regenerated axons and neuromuscular junctions was increased, muscle atrophy was reduced, the electrical response of the motor units was enhanced and the motor function of the injured upper limbs was greatly improved in rats with brachial plexus injury. These findings suggest that ISP and PAP4 promote the recovery of motor function after peripheral nerve injury in rats. The animal care and experimental procedures were approved by the Laboratory Animal Ethics Committee of Jinan University of China (approval No. 20111008001) in 2011.

Key words: axon, brachial plexus injury, crush injury, intracellular sigma peptide, motor function, PAP4, peripheral nerve, protection, regeneration, repair