中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (2): 459-464.doi: 10.4103/1673-5374.317990

• 原著:视神经损伤修复保护与再生 • 上一篇    

周围神经损伤后延期修复对靶肌肉运动终板空间分布的影响

  

  • 出版日期:2022-02-15 发布日期:2021-10-08

Effects of delayed repair of peripheral nerve injury on the spatial distribution of motor endplates in target muscle

Dong-Dong Li1, 2, #, Jin Deng1, #, Bo Jin1, Shuai Han1, Xin-Yi Gu1, Xue-Feng Zhou2, *, Xiao-Feng Yin1, *   

  1. 1Department of Trauma and Orthopedics, Peking University People’s Hospital, Beijing, China; 2Department of Orthopedics, PLA Strategic Support Force Medical Center, Beijing, China
  • Online:2022-02-15 Published:2021-10-08
  • Contact: Xiao-Feng Yin, PhD, xiaofengyin@bjmu.edu.cn; Xue-Feng Zhou, zhouxuefeng306@sina.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 82072162 (to XFY), 81971177; and the Natural Science Foundation of Beijing of China, No. 7192215 (to XFY). 

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摘要：

运动终板是运动神经元和骨骼肌进行信息交换的重要场所，在肌肉中以薄片形式分布。周围神经损伤后延期修复常由于运动终板变性而导致功能恢复效果不佳。实验横断小鼠胫神经，并在损伤后即刻、1个月以及3个月以可生物降解甲壳素导管桥接进行修复。通过活体小鼠尾静脉注射银环蛇毒素结合组织光透明技术，可见延期1或3个月修复小鼠骨骼肌中的运动终板依然呈现原有的片层状分布，但随着失神经时间延长，腓肠肌中运动终板的数量、每个薄片簇中运动终板数量和单个运动终板的成熟度逐渐降低，随着延期修复时间的延长单个运动终板的成熟度以及骨骼肌运动终板的总数均出现显著下降。表明延迟修复虽可恢复运动终板的空间分布，但对其均质性和数量方面存在不利影响。实验于2019年4月8日经北京大学人民医院伦理委员会批准（批准号2019PHC015）。

https://orcid.org/0000-0001-9932-642X (Xiao-Feng Yin): https://orcid.org/0000-0002-6678-216X (Xue-Feng Zhou)

关键词: 运动终板, 薄层簇, 周围神经损伤, 退化, 延迟修复, 组织光透明技术, 光片显微镜, 三维分布

Abstract: Motor endplates (MEPs) are important sites of information exchange between motor neurons and skeletal muscle, and are distributed in an organized pattern of lamellae in the muscle. Delayed repair of peripheral nerve injury typically results in unsatisfactory functional recovery because of MEP degeneration. In this study, the mouse tibial nerve was transected and repaired with a biodegradable chitin conduit, immediately following or 1 or 3 months after the injury. Fluorescent α-bungarotoxin was injected to label MEPs. Tissue optical clearing combined with light-sheet microscopy revealed that MEPs were distributed in an organized pattern of lamellae in skeletal muscle after delayed repair for 1 and 3 months. However, the total number of MEPs, the number of MEPs per lamellar cluster, and the maturation of single MEPs in gastrocnemius muscle gradually decreased with increasing denervation time. These findings suggest that delayed repair can restore the spatial distribution of MEPs, but it has an adverse effect on the homogeneity of MEPs in the lamellar clusters and the total number of MEPs in the target muscle. The study procedures were approved by the Animal Ethics Committee of the Peking University People’s Hospital (approval No. 2019PHC015) on April 8, 2019.

Key words: degeneration, delayed repair, lamellar cluster, light-sheet microscopy, motor endplates, peripheral nerve injury, three-dimensional distribution, tissue optical clearing

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