中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (7): 1617-1622.doi: 10.4103/1673-5374.330620

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

微小RNA有望作为改善创伤后骨骼肌生理功能的靶点

  

  • 出版日期:2022-07-15 发布日期:2022-01-18
  • 基金资助:
    国家自然科学基金项目(82072162,81971177);北京市自然科学基金项目(7192215)

MicroRNA is a potential target for therapies to improve the physiological function of skeletal muscle after trauma

Xin-Yi Gu1, 2, #, Bo Jin3, #, Zhi-Dan Qi1, 2, Xiao-Feng Yin1, 2, *   

  1. 1Department of Orthopedics and Traumatology, Peking University People’s Hospital, Beijing, China; 2Key Laboratory of Trauma and Neural Regeneration (Peking University), Beijing, China; 3Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, Jiangsu Province, China
  • Online:2022-07-15 Published:2022-01-18
  • Contact: Xiao-Feng Yin, MD, PhD, xiaofengyin@bjmu.edu.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 82072162, 81971177); and Beijing Municipal Natural Science Foundation of China, No. 7192215 (all to XFY).

摘要:

微小RNA有调节多种器官中离子通道的功能。基于作者既往研究可推测去神经支配后骨骼肌中高表达的miR-142a-3p可能通过类似的机制影响细胞的兴奋性。此次实验以慢病毒法过表达或敲低C2C12细胞中的miR-142a-3p,分化培养7d后记录全细胞电流变化。可见miR-142a-3p过表达可减少细胞的膜电容,并增大钾电流,减小钙通道电流;而敲低miR-142a-3p则减少钠通道电流。结果提示,miR-142a-3p的表达变化影响C2C12细胞中离子通道电流,进而表明其可能对肌细胞电生理产生影响。动物实验于2020年7月经北京大学动物伦理委员会批准(批准号LA2017128)。

https://orcid.org/0000-0001-9932-642X (Xiao-Feng Yin)

关键词: miR-142a-3p, C2C12细胞, 离子通道, 膜片钳, 去神经支配, 肌肉, 微小RNA, 钠, 钾,

Abstract: MicroRNAs can regulate the function of ion channels in many organs. Based on our previous study we propose that miR-142a-39, which is highly expressed in denervated skeletal muscle, might affect cell excitability through similar mechanisms. In this study, we overexpressed or knocked down miR-142a-3p in C2C12 cells using a lentivirus method. After 7 days of differentiation culture, whole-cell currents were recorded. The results showed that overexpression of miR-142a-3p reduced the cell membrane capacitance, increased potassium current density and decreased calcium current density. Knockdown of miR-142a-3p reduced sodium ion channel current density. The results showed that change in miR-142a-3p expression affected the ion channel currents in C2C12 cells, suggesting its possible roles in muscle cell electrophysiology. This study was approved by the Animal Ethics Committee of Peking University in July 2020 (approval No. LA2017128).

Key words: C2C12, denervation, ion channels, microRNA, miR-142a-3p, muscle, patch clamp, potassium, sodium, whole-cell currents