中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (11): 1819-1824.doi: 10.4103/1673-5374.170311

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

醋酸亮丙瑞林可促进损伤脊髓结构和功能的恢复s Quintanar1, *

  

  • 收稿日期:2015-08-20 出版日期:2015-12-07 发布日期:2015-12-07

Leuprolide acetate induces structural and functional recovery of injured spinal cord in rats.

Carmen Díaz Galindo1, Beatriz Gómez-González2, Eva Salinas3, Denisse Calderón-Vallejo1, Irma Hernández-Jasso1, Eduardo Bautista1,
J Luis Quintanar1, *   

  1. 1 Laboratory of Neurophysiology, Department of Physiology and Pharmacology, Universidad Autónoma de Aguascalientes, Aguascalientes, México
    2 Area of Neuroscience, Department of Reproductive Biology, CBS, Universidad Autónoma Metropolitana, Unidad Iztapalapa, México
    3 Laboratory of Immunology, Department of Microbiology, Centro de Ciencias Básicas, Universidad Autónoma de Aguascalientes, Aguascalientes, México
  • Received:2015-08-20 Online:2015-12-07 Published:2015-12-07
  • Contact: J Luis Quintanar, Ph.D.,jlquinta@correo.uaa.mx.
  • Supported by:

    This study was supported by a grant from CONACyT for scholarship 376921/246887.

摘要:

促性腺激素释放激素及其合成类似物促性腺激素释放激素激动剂醋酸亮丙瑞林有神经营养作用。为了检测醋酸亮丙瑞林是否能改善脊髓损伤大鼠脊髓形态、损伤中心小胶质细胞数量及运动、排尿功能、步态。在建立脊髓压迫伤大鼠模型后,给予大鼠肌肉注射醋酸亮丙瑞林或生理盐水5周。第5周时,醋酸亮丙瑞林干预大鼠运动功能恢复38%,步态明显改善,60%大鼠排尿功能有所改善。而生理盐水干预大鼠仅7%运动功能恢复,排尿功能无改善。更重要的是,醋酸亮丙瑞林干预降低了小胶质细胞免疫反应,并有扩大脊髓白质和灰质面积的趋势。因此,我们认为醋酸亮丙瑞林有修复脊髓损伤的潜力。

关键词: 神经再生, 脊髓损伤, 醋酸亮丙瑞林, 促性腺激素释放激素, 神经营养因子, 小胶质细胞, 排尿反射, 步态, 炎症

Abstract:

Gonadotropin-releasing hormone (GnRH) and its synthetic analog leuprolide acetate, a GnRH agonist, have neurotrophic properties. This study was designed to determine whether administration of leuprolide acetate can improve locomotor behavior, gait, micturition reflex, spinal cord morphology and the amount of microglia in the lesion epicenter after spinal cord injury in rats. Rats with spinal cord compression injury were administered leuprolide acetate or saline solution for 5 weeks. At the 5th week, leuprolide acetate-treated rats showed locomotor activity recovery by 38%, had improvement in kinematic gait and exhibited voiding reflex recovery by 60%, as compared with the 1st week. By contrast, saline solution-treated rats showed locomotor activity recovery only by 7%, but voiding reflex did not recover. More importantly, leuprolide acetate treatment reduced microglial immunological reaction and induced a trend towards greater area of white and gray matter in the spinal cord. Therefore, leuprolide acetate has great potential to repair spinal cord injury.

Key words: nerve regeneration, spinal cord injury, leuprolide acetate, gonadotropin-releasing hormone, neurotrophic factor, microglia, micturition reflex, gait, inflammation, neural regeneration