中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (7): 1252-1257.doi: 10.4103/1673-5374.301020

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

极低频电磁场能促进脑缺血大鼠海马中神经发生和认知功能

  

  • 出版日期:2021-07-15 发布日期:2021-01-07
  • 基金资助:

    中国国家自然科学基金项目(81201513

Extremely low frequency electromagnetic fields promote cognitive function and hippocampal neurogenesis of rats with cerebral ischemia

Qiang Gao1, 2, 3, Aaron Leung2, 3, *, Yong-Hong Yang1, 2, 4, Benson Wui-Man Lau4, Qian Wang1, 2, Ling-Yi Liao1, Yun-Juan Xie1, Cheng-Qi He1, 2, *   

  1. 1 Department of Rehabilitation Medicine, West China Hospital of Sichuan University, Chengdu, Sichuan Province, China;  2 Institute of Disaster Management and Reconstruction, Sichuan University–The Hong Kong Polytechnic University, Chengdu, Sichuan Province, China;  3 Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, China;  4 Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region, China
  • Online:2021-07-15 Published:2021-01-07
  • Contact: Aaron Leung, PhD, aaron.leung@polyu.edu.hk; Cheng-Qi He, MD, hxkfhcq@126.com.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 81201513 (to QG).

摘要:

极低频电磁场虽能改善阿尔茨海默病大鼠的学习和记忆障碍,但其对脑缺血的作用尚有待研究。实验建立大脑中动脉闭塞再灌注脑缺血大鼠模型。于造模后24h起,以50 Hz1 mT极低频电磁场进行全身治疗,连续治疗28d。结果发现,经极低频电磁场治疗后,大鼠Morris水迷宫定位航行测试中游泳距离和潜伏期缩短,在探索试验穿越平台的次数和目标象限停留的时间增加,海马颗粒下层中代表新生神经元的BrdU+/NeuN+细胞数量明显增加,且海马颗粒下层中Notch信号通路的关键因子Notch1NotIHes5蛋白的表达上调。表明极低频电磁场可增强脑缺血大鼠海马中的神经发生,且其作用可能是通过影响Notch信号通路实现的。实验于2019年35日经四川大学伦理委员会批准,批准号2019255A。

https://orcid.org/0000-0003-3603-749X (Qiang Gao)

关键词: 电磁场, 卒中, 脑缺血, 认知功能, 信号通路, 神经发生, 可塑性, 修复

Abstract: Extremely low frequency electromagnetic fields (ELF-EMF) can improve the learning and memory impairment of rats with Alzheimer’s disease, however, its effect on cerebral ischemia remains poorly understood. In this study, we established rat models of middle cerebral artery occlusion/reperfusion. One day after modeling, a group of rats were treated with ELF-EMF (50 Hz, 1 mT) for 2 hours daily on 28 successive days. Our results showed that rats treated with ELF-EMF required shorter swimming distances and latencies in the Morris water maze test than those of untreated rats. The number of times the platform was crossed and the time spent in the target quadrant were greater than those of untreated rats. The number of BrdU+/NeuN+ cells, representing newly born neurons, in the hippocampal subgranular zone increased more in the treated than in untreated rats. Up-regulation in the expressions of Notch1, Hes1, and Hes5 proteins, which are the key factors of the Notch signaling pathway, was greatest in the treated rats. These findings suggest that ELF-EMF can enhance hippocampal neurogenesis of rats with cerebral ischemia, possibly by affecting the Notch signaling pathway. The study was approved by the Institutional Ethics Committee of Sichuan University, China (approval No. 2019255A) on March 5, 2019. 

Key words: cerebral ischemia, cognitive function, electromagnetic fields, hippocampus, neurogenesis, plasticity, repair, signaling pathway, stroke, rat